Characterization of Human Senescent Cell Biomarkers for Clinical Trials.

ABSTRACT: There is an increasing need for biomarkers of senescent cell burden to facilitate the selection of participants for clinical trials. p16^Ink4a is encoded by the CDKN2A locus, which produces five variant transcripts in humans, two of which encode homologous p16 proteins: p16^Inka4a, encoded by p16_variant 1, and p16ɣ, encoded by p16_variant 5. While distinct quantitative polymerase chain reaction primers can be designed for p16_variant 5, primers for p16_variant 1 also measure p16_variant 5 (p16_variant 1 + 5). In a recent clinical trial evaluating the effects of the senolytic combination, dasatinib + quercetin (D + Q), on bone metabolism in postmenopausal women, we found that women in the highest tertile for T-cell expression of p16_variant 5 had the most robust skeleta

SUBMITTER: Farr JN 

PROVIDER: S-EPMC12073900 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature