An additional replication origin causes cell cycle specific DNA replication fork speed.

ABSTRACT: Replication fork speed (RFS) in Escherichia coli has long been considered constant throughout the replication and cell cycles. In wild-type cells, the circular chromosome is duplicated bidirectionally from oriC, yielding two replication forks that converge at the ter region. Under slow-growth conditions, cells are smaller at initiation than at termination, so DNA replication consumes a larger fraction of cellular resources early in the cell cycle. To challenge this paradigm, we analyzed an E. coli strain with an additional ectopic copy of oriC-designated oriX-inserted midway along the left replichore. In this mutant, replication initiates simultaneously from both oriC and oriX, resulting in four active replication forks early in the cycle. S

SUBMITTER: Skovgaard O 

PROVIDER: S-EPMC12075136 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature