Emergence of an XDR <i>Klebsiella pneumoniae</i> ST5491 strain co-harboring NDM-5, MCR-1.1, tmexCD1-toprJ1, and a novel plasmid carrying CTX-M-15.

ABSTRACT:

Objective

The rapid emergence of antimicrobial resistance (AMR) in Klebsiella pneumoniae poses a significant global health threat. The study aimed to analyze and describe the genomic architecture and resistance mechanisms of an extensively drug-resistant (XDR) K. pneumoniae isolate, KP09, by focusing on plasmids that harbor multiple resistance genes, including tmexCD1-toprJ1, bla_CTX-M-15 , bla_NDM-5 , and mcr-1.1.

Methods

The KP09 strain, isolated from a clinical sample, was subjected to antimicrobial susceptibility testing and conjugation experiments. Whole-genome sequencing with both long- and short-read methods facilitated hybrid assembly for complete genome reconstruction. Bioinformatics analyses identified r

SUBMITTER: Fang Y 

PROVIDER: S-EPMC12075367 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature