FOXM1 boosts glycolysis by upregulating SQLE to inhibit anoikis in breast cancer cells.

ABSTRACT:

Background

Resisting anoikis is a prerequisite for cancer to spread and invade and a major cause of cancer-related deaths. Yet, the intricate mechanisms of how cancer cells evade anoikis remain largely unknown. There is a significant need to explore how these mechanisms play out in breast cancer (BC).

Methods

Bioinformatics analysis revealed the expression levels of SQLE and FOXM1 in BC tissue, along with their correlation. The enrichment pathways of SQLE were also explored. qPCR detected the expression of SQLE and FOXM1 in BC cells. CCK-8 assessed cell viability, while flow cytometry measured anoikis. Western blot was employed to examine the protein expression of key genes in glycolytic metabolism and apoptosis-related proteins. Extracellular acidification rate was quantifi

SUBMITTER: Xu M 

PROVIDER: S-EPMC12075401 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature