Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation.

ABSTRACT: The predatory bacterium, Myxococcus xanthus, kills its prey by contact, using a putative Tight Adherence pilus, known as the Kil system, along with a protein complex resembling the basal body a type-III secretion system, named the "needleless" T3SS*. In this work, we provide direct evidence that Myxococcus polymerizes a Kil pilus at the prey contact site, which is constituted by the major pilin KilP. We also genetically demonstrate that the predation function of this pilus is linked to four different minor pilin complexes, which work in specific combinations to detect and kill phylogenetically diverse bacterial species. Structural models of the Kil pilus suggest that these minor pilin complexes form interchangeable "Tips", exposing variable domains at the extremity of the pilus to interact

SUBMITTER: Herrou J 

PROVIDER: S-EPMC12075869 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature