Identification of the <i>Staphylococcus aureus</i> endothelial cell surface interactome by proximity labeling.

ABSTRACT: Virulence strategies of pathogens depend on interaction with host cells. The binding and activation of receptors located on the plasma membrane are crucial for the attachment to or pathogen internalization by host cells. Identifying host cell receptors is often difficult, and hence, the identity of many proteins that play important roles during host-pathogen interaction remains elusive. We developed a novel proximity labeling approach by decorating the opportunistic pathogen Staphylococcus aureus with ascorbate peroxidase 2. Upon addition of hydrogen peroxide, the peroxidase initiates proximity biotinylation of S. aureus host interaction partners, thereby enabling the identification of these proteins. Here, we demonstrate an endothelial cell surface interactome of 305 protein

SUBMITTER: Ruhling M 

PROVIDER: S-EPMC12077161 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature