Ultrasensitive and highly specific detection of the <i>Brucella</i> genus and <i>B</i>. <i>melitensis</i> by CRISPR/Cas12b-multiple cross displacement amplification technique.

ABSTRACT: Brucellosis is caused by members of the Brucella spp. and remains one of the world's major zoonotic diseases. Brucella melitensis (B. melitensis) as the most contagious Brucella species cannot be ignored as an essential source of infection for brucellosis, especially in countries/regions dominated by animal husbandry. Thus, the identification of the Brucella genus and B. melitensis is crucial for rapid diagnosis of brucellosis to control disease transmission and clinical treatment. Here, we developed the CRISPR/Cas12b nuclease combined with a multiple cross displacement amplification (MCDA) assay (CRISPR-MCDA) for highly specific and sensitive detection of Brucella genus and B. melitensis in clinical applications. Two sets of specific

SUBMITTER: Mao S 

PROVIDER: S-EPMC12077202 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature