Biochemical mapping reveals a conserved heme transport mechanism via CcmCD in System I bacterial cytochrome <i>c</i> biogenesis.

ABSTRACT: Heme is a redox-active cofactor for essential processes across all domains of life. Heme's redox capabilities are responsible for its biological significance but also make it highly cytotoxic, requiring tight intracellular regulation. Thus, the mechanisms of heme trafficking are still not well understood. To address this, the bacterial cytochrome c biogenesis pathways are being developed into model systems to elucidate mechanisms of heme trafficking. These pathways function to attach heme to apocytochrome c, which requires the transport of heme from inside to outside of the cell. Here, we focus on the System I pathway (CcmABCDEFGH) which is proposed to function in two steps: CcmABCD transports heme across the membrane and attaches it to CcmE. HoloCcmE then transports heme to

SUBMITTER: Kreiman AN 

PROVIDER: S-EPMC12077264 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature