Finding antibodies in cryo-EM maps with CrAI.

ABSTRACT:

Motivation

Therapeutic antibodies have emerged as a prominent class of new drugs due to their high specificity and their ability to bind to several protein targets. Once an initial antibody has been identified, its design and characteristics are refined using structural information, when it is available. Cryo-EM is currently the most effective method to obtain 3D structures. It relies on well-established methods to process raw data into a 3D map, which may, however, be noisy and contain artifacts. To fully interpret these maps the number, position, and structure of antibodies and other proteins present must be determined. Unfortunately, existing automated methods addressing this step have limited accuracy, require additional inputs and high-resolution maps, and exhibit long running

SUBMITTER: Mallet V 

PROVIDER: S-EPMC12077295 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature