Real-time monitoring of cellular superoxide anion release in THP-1 cells using a catalytically amplified superoxide dismutase-based microbiosensor.

ABSTRACT: Reactive oxygen species (ROS) including the superoxide anion (O₂^•-) are typically studied in cell cultures using fluorescent dyes, which provide only discrete single-point measurements. These methods lack the capabilities for assessing O₂^•- kinetics and release in a quantitative manner over long monitoring times. Herein, we present the fabrication and application of an electrochemical biosensor that enables real-time continuous monitoring of O₂^•- release in cell cultures for extended periods (> 8 h) using an O₂^•- specific microelectrode. To achieve the sensitivity and selectivity requirements for cellular sensing, we developed a biohybrid system consisting of superoxide dismutase (SOD) and Ti₃

SUBMITTER: Deshpande AS 

PROVIDER: S-EPMC12077743 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature