Human naive B cells show evidence of anergy and clonal redemption following vaccination.

ABSTRACT: In an era of predicted emerging pandemics, the production of effective vaccines may require an in-depth understanding of the biology of human naive B (B_N) cells. Here we provide evidence that the majority of B_N cells expressed CD73, an ecto-5'-nucleotidase often associated with immune cell suppression, and demonstrated features of anergy, including an IgM^lowIgD⁺ surface phenotype, reduced calcium flux in response to IgM crosslinking, and increased PTEN expression. Analysis of antibody sequences encoded by the inherently autoreactive V_H4-34 heavy chain produced by plasmablasts seven days following influenza vaccination showed that in younger but not in older individuals, anergic B_N cells provided a reservoir of B cells capabl

SUBMITTER: Dizon BLP 

PROVIDER: S-EPMC12078529 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature