Adult human heart extracellular matrix improves human iPSC-CM function via mitochondrial and metabolic maturation.

ABSTRACT: Myocardial infarction can lead to the loss of billions of cardiomyocytes, and while cell-based therapies are an option, immature nature of in vitro-generated human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iCMs) is a roadblock to their development. Existing iPSC differentiation protocols don't go beyond producing fetal iCMs. Recently, adult extracellular matrix (ECM) was shown to retain tissue memory and have some success driving tissue-specific differentiation in unspecified cells in various organ systems. Therefore, we focused on investigating the effect of adult human heart-derived extracellular matrix (ECM) on iPSC cardiac differentiation and subsequent maturation. By preconditioning iPSCs with ECM, we tested whether creating cardiac environments around iPSCs would d

SUBMITTER: Gulberk Ozcebe S 

PROVIDER: S-EPMC12080356 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature