Widespread discordance between mRNA expression, protein abundance and <i>de novo</i> lipogenesis activity in hepatocytes during the fed-starvation transition.

ABSTRACT: The mammalian liver plays a critical role in maintaining metabolic homeostasis during fasting and feeding. Liver function is further shaped by sex dimorphism and zonation of hepatocytes. To explore how these factors interact, we performed deep RNA-sequencing and label-free proteomics on periportal and pericentral hepatocytes isolated from male and female mice under fed and starved conditions. We developed a classification system to assess protein-mRNA relationship and found that gene products (mRNA or protein) for most zonation markers showed strong concordance between mRNA and protein. Although classical growth hormone regulated sex-biased gene products also exhibited concordance, ~60% of sex-biased gene products showed protein-level enrichment without corresponding mRNA differences. In c

SUBMITTER: Landgraf A 

PROVIDER: S-EPMC12080948 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature