Integrated analysis of molecular atlases unveils modules driving developmental cell subtype specification in the human cortex.

ABSTRACT: Human brain development requires generating diverse cell types, a process explored by single-cell transcriptomics. Through parallel meta-analyses of the human cortex in development (seven datasets) and adulthood (16 datasets), we generated over 500 gene co-expression networks that can describe mechanisms of cortical development, centering on peak stages of neurogenesis. These meta-modules show dynamic cell subtype specificities throughout cortical development, with several developmental meta-modules displaying spatiotemporal expression patterns that allude to potential roles in cell fate specification. We validated the expression of these modules in primary human cortical tissues. These include meta-module 20, a module elevated in FEZF2⁺ deep layer neurons that includes TSHZ3, a

SUBMITTER: Nano PR 

PROVIDER: S-EPMC12081304 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature