Japanese encephalitis virus-associated human microglia induce cell death of human microvascular endothelial cells in receptor-independent infection.

ABSTRACT:

Introduction

The neurotropic virus Japanese encephalitis virus invades the human central nervous system, inducing neuroinflammation and further disruption of the blood-brain barrier. JEV interacts with various cell types of the blood-brain barrier including the endothelial cells. The present work aims to investigate impact of receptor-dependent and independent infection of human microvascular endothelial cells by Japanese encephalitis virus.

Methods

Receptor-dependent infection was achieved using cell-free virus while receptor-independent infection was by co-culture of microvascular endothelial cells with virus-associated microglia.

Results

While both receptor-dependent and independent infections of human microvascular endothelial cells led to virus propagation, only

SUBMITTER: Fellay I 

PROVIDER: S-EPMC12081440 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature