Identification and expression analysis of <i>PAL</i> genes related to chlorogenic acid synthesis in <i>Vaccinium dunalianum</i> Wight.

ABSTRACT: Phenylalanine ammonia-lyase (PAL) is the first key enzyme in the metabolic pathway of phenylpropanoid. Chlorogenic acid (CGA) is an important secondary metabolite in the phenylpropanoid metabolic pathway of plants. However, the role of the PAL gene in CGA biosynthesis in Vaccinium dunalianum Wight is still unclear. In this study, seven PAL genes (VdPAL1-VdPAL7) were cloned and analyzed through full-length transcriptome sequencing combined with reverse transcription polymerase chain reaction (RT-PCR) amplification in V. dunalianum. Bioinformatics analysis revealed that the seven VdPAL genes contain complete open reading frames (1,860-2,148 bp) encoding hydrophilic proteins of 619-715 amino acids, with molecular weights ranging from 67.44 to 7

SUBMITTER: An X 

PROVIDER: S-EPMC12081449 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature