New insights on poly(<i>cis</i>-1,4-isoprene) rubber degradation through enzymatic kinetics and process improvement.

ABSTRACT: Latex clearing protein (Lcp) is a crucial enzyme in the oxidative degradation of poly(cis-1,4-isoprene), the main component of natural rubber (NR). Despite significant biochemical advances, to date, the kinetic behavior of Lcp from Streptomyces sp. K30 (Lcp_K30) has not been characterized, limiting the efficiency of NR conversion. In this work, Lcp_K30 was produced in Escherichia coli BL21 (DE3) + p4782.1::lcp _K30 with L-rhamnose as the inducer, yielding 6.05 mg/L of purified protein. Kinetic assays demonstrated a positive correlation between the initial reaction rate and poly(cis-1,4-isoprene) concentration, reaching a maximum rate of 7.05 nmol O₂/min at the enzyme's saturation point, corresponding to 5 μg Lcp/mg

SUBMITTER: Guajardo-Flores C 

PROVIDER: S-EPMC12081455 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature