Characterisation of the periodontal proteome in gingival crevicular fluid and saliva using SWATH-MS.

ABSTRACT:

Introduction

Proteomic techniques are useful to analyse the periodontal proteome in gingival crevicular fluid (GCF) and saliva. However, few investigations have assessed and compared the GCF and salivary proteomes. Therefore, this research aims to analyse the proteome structure and compare protein expression in these fluids between individuals with periodontal health and those with periodontitis.

Methods

GCF and saliva were collected from 44 periodontally healthy subjects and 41 with periodontitis (stages III-IV). Samples were analysed using sequential window acquisition of all theoretical mass spectra (SWATH-MS), and proteins were identified employing the UniProt database. The periodontal proteome structure was assessed using principal component analysis (PCA). Differential

SUBMITTER: Blanco-Pintos T 

PROVIDER: S-EPMC12081464 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature