During recombinase-mediated homology recognition RecQ helicases inhibit formation of toxic long-lived D-loops that could promote genomic instability.

ABSTRACT: Mutations in RecQ family helicases underlie human genetic disorders associated with genomic instability and cancer predisposition, but questions remain about how properly functioning RecQ reduces these deleterious effects. Importantly, some of the deleterious effects may result from incorrect repair of DNA double-strand breaks (DSBs) by recombinase proteins. Displacement loops (D-loops) are three-strand intermediates formed by recombinases during repair of DSB. RecQ helicases might enhance genome stability by disassembling incorrect recombinase-mediated D-loops formed between mismatched sequences and/or between short regions of accidental homology. We used bulk FRET and gel electrophoresis assays to probe the effects of RecQ family helicases in the context of ongoing recombinase-mediated D

SUBMITTER: Danilowicz C 

PROVIDER: S-EPMC12082448 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature