Celastrol protects against diabetic nephropathy by modulating immune-related pathways: a bioinformatics and experimental validation.

ABSTRACT:

Objectives

Celastrol has shown therapeutic effects in diabetic nephropathy (DN). This study aimed to elucidate its underlying mechanisms through bioinformatics analysis and experimental validation.

Methods

Differentially expressed genes (DEGs) between DN and control groups were obtained from GSE30122 and GSE30528 datasets. Target genes of Celastrol were collected from relevant biological databases and intersected with the DEGs. Functional enrichment analysis was conducted to explore the associated biological processes. Immune cell infiltration in DN was analyzed, and a Lasso regression model was constructed to identify DN-associated gene markers with diagnostic potential. The binding affinity of celastrol to target proteins was evaluated using molecular docking. Additionally

SUBMITTER: Wang X 

PROVIDER: S-EPMC12082535 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature