Macrophage sensitivity to bexmarilimab-induced reprogramming is shaped by the tumor microenvironment.

ABSTRACT:

Background

Tumor-associated macrophages (TAMs) adapt to the tumor microenvironment (TME), either aiding cancer eradication or promoting tumor growth and immune evasion. To manipulate TAMs therapeutically, a deep understanding of their interaction with the TME is essential. This study explores the responsiveness of TMEs to bexmarilimab, a macrophage reprogramming therapy showing clinical benefit in various solid tumors.

Methods

We exploited a breast cancer patient-derived explant culture (PDEC) model to characterize bexmarilimab responses in both tumor and adjacent cancer-free tissues by RNA sequencing and multiplex cytokine profiling. Using single-cell RNA sequencing, spatial transcriptomics, and conditioned media treatment, we further investigated the effects of Clever-1+ m

SUBMITTER: Rannikko JH 

PROVIDER: S-EPMC12083384 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature