Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for Biomimetic Long-Term Cell Cultures.

ABSTRACT: Surface modification of polydimethylsiloxane (PDMS) for organ-on-a-chip (OOC) systems is fundamental for the success of cell physiological assays. Although UV light is commonly used for this purpose, surface chemical modifications are only temporary. To overcome these limitations, an alternative approach is proposed: a physicochemical modification using ozone and the heterofunctional crosslinker sulfo-SANPAH (SS). This simple one-step approach is carried out on PDMS microchannels of OOC platforms. A broad physicochemical characterization based on spectroscopy and microscopy techniques  confirms successful modification of the PDMS surface. The PDMS surface contact angle decreases after addition of SS (from 101.4° to 79.9°) but decreases as low as 20.8° after extracellular matrix deposition,

SUBMITTER: Perez-Calixto M 

PROVIDER: S-EPMC12083443 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature