The evolution of AKAPs and emergence of PKA isotype selective anchoring determinants.

ABSTRACT: Cyclic AMP is a versatile signaling molecule utilized throughout the eukaryotic domain. A frequent use is to activate protein kinase A (PKA), a serine/threonine kinase that drives many physiological responses. Spatiotemporal organization of PKA occurs though association with A-kinase anchoring proteins (AKAPs). Sequence alignments and phylogenetic analyses trace the evolution of PKA regulatory (R) and catalytic (C) subunits and AKAPs from the emergence of metazoans. AKAPs that preferentially associate with the type I (RI) or type II (RII) regulatory subunits diverged at the advent of the vertebrate clade. Type I PKA anchoring proteins including smAKAP contain an FA motif at positions 1 and 2 of their amphipathic binding helices. Fluorescence recovery after photobleaching measurements indic

SUBMITTER: Falcone JI 

PROVIDER: S-EPMC12083921 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature