In vitro studies of human erythropoiesis using a 3D silk-based bone marrow model that generates erythroblastic islands.

ABSTRACT:

Abstract

The pursuit of ex vivo erythrocyte generation has led to the development of various culture systems that simulate the bone marrow microenvironment. However, these models often fail to fully replicate the hematopoietic niche's complex dynamics. In our research, we use a comprehensive strategy that emphasizes physiological red blood cell (RBC) differentiation using a minimal cytokine regimen. A key innovation in our approach is the integration of a 3-dimensional (3D) silk-based scaffold engineered to mimic both the physical and chemical properties of human bone marrow. This scaffold facilitates critical macrophage-RBC interactions and incorporates fibronectin functionalization to support the formation of erythroblastic island (EBI)-like niches. We observed diverse stages of

SUBMITTER: Di Buduo CA 

PROVIDER: S-EPMC12083923 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature