Single-cell transcriptomics reveals metabolic remodeling and functional specialization in the immune microenvironment of bone tumors.

ABSTRACT:

Objective

To investigate the metabolic remodeling and functional specialization of immune cells within the tumor microenvironment (TME) of bone tumors, including Ewing's sarcoma, osteosarcoma, and giant cell tumor of bone, through high-resolution single-cell RNA sequencing (scRNA-seq) analysis.

Methods

Immune cells were isolated from 13 bone tumor samples and profiled via scRNA-seq to delineate cellular compositions, metabolic adaptations, and intercellular communication networks. Differential gene expression analysis, metabolic pathway enrichment, and pseudotime trajectory inference were employed to characterize functional states and differentiation processes of immune cell subsets.

Results

We identified 12 major immune cell clusters with distinct functional and meta

SUBMITTER: Chen J 

PROVIDER: S-EPMC12084933 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature