M6A-lncRNA landscape highlights reduced levels of m6A modification in glioblastoma as compared to low-grade glioma.

ABSTRACT:

Background

Efforts to understand the interplay between m6A (N6-methyladenosine) modification and long non-coding RNAs (lncRNAs) in the pathogenesis of various diseases, including cancer, have recently attracted considerable attention.

Methods

Herein, we profiled epitranscriptome-wide m6A modifications within lncRNAs at single m6A site resolution across different grades of gliomas (Glioblastomas (GB): n = 17, Low grade gliomas (LGG): n = 9) using direct RNA long-read sequencing.

Results

Our analysis demonstrated that, 1) 98.5% of m6A-modified RRACH motifs were present within mRNA transcripts, while only 1.16% were conspicuous within lncRNAs. Importantly, LGGs exhibited a higher m6A abundance (23.73%) compared to the GB transcriptome (15.84%). 2) The m6A profiles of lnc

SUBMITTER: Steponaitis G 

PROVIDER: S-EPMC12085027 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature