In vitro, genomic characterization and pre-clinical evaluation of a new thermostable lytic Obolenskvirus phage formulated as a hydrogel against carbapenem-resistant Acinetobacter baumannii.

ABSTRACT: The urgent threat of carbapenem-resistant Acinetobacter baumannii (CRAB) necessitates the development of new antimicrobial strategies. Bacteriophage (phage) therapy is one of the most promising alternative strategies that can be implemented to combat multidrug-resistant (MDR) bacterial infections. Herein, an A. baumannii phage VB_AB_Acb75 that exhibited lytic activity against 6 CRAB isolates (21.43%) with stability at up to 70 °C, pH 2-12, and high concentrations of organic solvents was isolated and characterized. The transmission electron microscope (TEM) detected a tailed phage with an icosahedral head and contractile tail (myoviral morphotype). The Oxford nanopore sequencing results showed an A. baumannii phage genome size of 45,487 bp, a G + C content of 38%, and 42 open reading frames

SUBMITTER: Sherif MM 

PROVIDER: S-EPMC12085604 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature