Single cell transcriptomics correlate avian coronavirus prime vaccination efficacy with antigen-presenting cell preference.

ABSTRACT: Biosafe and effective vaccines are urgently needed for the prevention and control of avian infectious bronchitis virus (IBV), the first coronavirus to be discovered, despite extensive vaccination for decades. However, their development has been hindered by our limited understanding of prime vaccination, which is crucial for rational vaccine design. Here, we constructed in vivo dynamic single-cell resolution blood immune landscapes of chickens immunized with live-attenuated or inactivated IBV. Bioinformatic analysis together with in vivo examination revealed that live-attenuated and inactivated vaccines reshaped lymphocytes and led to identical compositions through different mechanisms. Inactivated vaccines activate T lymphocytes through dendritic cells with subsequent T lymphocyte-dependen

SUBMITTER: Li X 

PROVIDER: S-EPMC12085680 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature