Transcriptomics Uncovers Key Genes for Photodynamic Killing on Trichosporon asahii Biofilms.

ABSTRACT:

Background

The escalating threat of antifungal resistance stemming from Trichosporon asahii (T. asahii) biofilms necessitates the pursuit of innovative therapeutic strategies. Among these approaches, 5-aminolevulinic acid (ALA) photodynamic therapy (PDT), an emerging therapeutic modality, has exhibited promising potential in eradicating T. asahii biofilms.

Methods

The inhibitory activity was evaluated by confocal laser scanning microscopy. To delve deeper into the efficacy of ALA-PDT in eliminating T. asahii biofilms, we conducted a comprehensive transcriptional analysis utilizing transcriptome sequencing.

Results

ALA-PDT demonstrated a profound inhibitory effect on the viability of T. asahii biofilms. Our investigation unveiled 2720 differentially expressed genes fol

SUBMITTER: Luo W 

PROVIDER: S-EPMC12086123 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature