RNA polymerase I is essential for driving the formation of 3D genome in early embryonic development in mouse, but not in human.

ABSTRACT:

Background

Three-dimensional (3D) chromatin architecture undergoes dynamic reorganization during mammalian gametogenesis and early embryogenesis. While mouse studies have shown species-specific patterns as well as mechanisms underlying de novo organization, these remain poorly characterized in humans. Although RNA polymerases II and III have been shown to regulate chromatin structure, the potential role of RNA polymerase I (Pol I), which drives ribosomal RNA production, in shaping 3D genome organization during these developmental transitions has not been investigated.

Methods

We employed a modified low-input in situ Hi-C approach to systematically compare 3D genome architecture dynamics from gametogenesis through early embryogenesis in human and mouse. Complementary Smart-se

SUBMITTER: Hou C 

PROVIDER: S-EPMC12087037 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature