Spatiotemporal single-cell analysis elucidates the cellular and molecular dynamics of human cornea aging.

ABSTRACT:

Background

The human cornea is a transparent and uniquely ordered optical-biological system. Precise coordination of its cellular mechanisms is essential to maintain its transparency and functionality. However, the spatial, cellular and molecular architecture of the human cornea and its intercellular interactions during aging have not been elucidated.

Methods

We performed single-cell RNA sequencing (scRNA-seq) and single-cell SpaTial Enhanced REsolution Omics-sequencing (scStereo-seq) analysis in corneal tissue from eight eyes of donors aged 33-88 years to elucidate the spatiotemporal cellular and molecular dynamics of human cornea aging. Immunofluorescence staining and Western blotting were performed to validate the findings.

Results

Spatiotemporal single-cell analys

SUBMITTER: Jiang D 

PROVIDER: S-EPMC12087038 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature