Quantitative spatial analysis of chromatin biomolecular condensates using cryoelectron tomography.

ABSTRACT: Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficulties in visualizing the condensate interior at high resolution. Here, we analyzed the structure of biochemically reconstituted chromatin condensates through cryoelectron tomography. We found that traditional blotting methods of sample preparation were inadequate, and high-pressure freezing plus focused ion beam milling was essential to maintain condensate integrity. To identify densely packed molecules within the condensate, we integrated deep learning-based segmentation with context-aware template matching. Our approaches were developed on chromatin condensates and were also effective on cond

SUBMITTER: Zhou H 

PROVIDER: S-EPMC12088439 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature