Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines.

ABSTRACT:

Background

Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer.

Methods

GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay.

Results

GIRK1 mRNA was expressed in three of six small cell lung cancer (SCLC) cell lines, and either GIRK2, 3 or 4 mRNA expression was detected in al

SUBMITTER: Plummer HK 

PROVIDER: S-EPMC1208863 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature