Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development.

ABSTRACT: Vibrio natriegens is a fast-growing microbial workhorse with high potential for biotechnological applications. However, handling the bacterium in batch processes is challenging due to its high overflow metabolism and mixed acid formation under microaerobic conditions. For early process development, technologies enabling small-scale fed-batch cultivation of V. natriegens Vmax are needed. In this study, fed-batch cultivations in 96-well microtiter plates were successfully online-monitored for the first time with a µTOM device. Using the online-monitored oxygen transfer rate, a scale up to membrane-based fed-batch shake flasks was performed. The overflow metabolism was efficiently minimized by choosing suitable feed rates, and mixed acid formation was prevented. A glucose soft sensor using th

SUBMITTER: Luchtrath C 

PROVIDER: S-EPMC12089209 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature