An aggregation-induced conformation locking strategy facilitates the activation of lipase biocatalyst.

ABSTRACT: Lipase represents one of the most important industrial biocatalysts, with a global market value of $590.5 million by 2020. However, their catalytic efficiency is often hindered by a closed "lid" conformation. Here, we present an aggregation-induced conformation locking strategy that enables the facile synthesis of highly activated lipase hybrid biocatalysts. Lipase is self-activated into an open-lid conformation via solvent-mediated aggregation, followed by conformational locking within a two-dimensional metal-organic framework (MOF). The resulting MOF biocatalyst provides high accessibility to the locked lipase aggregates through its long-range ordered pore channels, achieving a hydrolytic efficiency 5.30 times greater than that of native lipase. To the best of our knowledge, this represe

SUBMITTER: Huang A 

PROVIDER: S-EPMC12089486 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature