Effect of SAM-Dependent Methyltransferases from <i>Halomonas</i> sp. YLGW01 on Phospholipid Fatty Acids Composition and Production of Polyhydroxalkanoates in <i>Escherichia coli</i>.

ABSTRACT: The bacterial membrane changes in response to growth conditions such as salt concentration, temperature, and growth inhibitors. As membrane fatty acid related genes are important in controlling the membrane composition, we studied two genes i.e. cyclopropane fatty acid synthase (cfa) and cis-vaccinate 11-methyltransferase (ufaM) that designated to S-adenosylmethionine (SAM)-dependent methyltransferase derivatives from Halomonas sp. YLGW01. These two genes are related to a response to external growth conditions by controlling the fluidity of membrane changes. The role of these two genes (cfa and ufaM) was accessed by overexpression in Escherichia coli and results showed an evident increase in the levels of cyclic fatty acid and detection of f

SUBMITTER: Choi TR 

PROVIDER: S-EPMC12089945 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature