Matrix Stiffness Regulates the Osteogenic Differentiation of hPDLSCs via DNA Methylation.

ABSTRACT:

Objectives

This study aimed to examine the influence of matrix stiffness on osteogenic differentiation via epigenetic mechanisms in human periodontal ligament stem cells (hPDLSCs), with implications for understanding orthodontic tooth movement.

Materials and methods

hPDLSCs were cultured on substrates with varying stiffness (soft and stiff). Dot blot and immunofluorescence techniques were employed to measure global DNA methylation levels. RT-qPCR and alkaline phosphatase (ALP) activity assays were conducted to assess differences in DNA methylation and osteogenic potential. Additionally, ELISA was used to quantify DNA methyltransferase content and activity.

Results

hPDLSCs on stiffer substrates exhibited increased 5-methylcytosine (5-mC) and higher global DNA methylati

SUBMITTER: Ding R 

PROVIDER: S-EPMC12090244 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature