Lactate dehydrogenase A-coupled NAD⁺ regeneration is critical for acute myeloid leukemia cell survival.

ABSTRACT:

Background

Enhanced glycolysis plays a pivotal role in fueling the aberrant proliferation, survival and therapy resistance of acute myeloid leukemia (AML) cells. Here, we aimed to elucidate the extent of glycolysis dependence in AML by focusing on the role of lactate dehydrogenase A (LDHA), a key glycolytic enzyme converting pyruvate to lactate coupled with the recycling of NAD⁺.

Methods

We compared the glycolytic activity of primary AML patient samples to protein levels of metabolic enzymes involved in central carbon metabolism including glycolysis, glutaminolysis and the tricarboxylic acid cycle. To evaluate the therapeutic potential of targeting glycolysis in AML, we treated AML primary patient samples and cell lines with pharmacological inhibitors of LDHA and

SUBMITTER: Erdem A 

PROVIDER: S-EPMC12090514 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature