PTripleTREP - A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus.

ABSTRACT:

Background

Recombinant proteins facilitate and contribute to detailed studies of the virulence mechanisms and pathophysiology of the major human pathogen Staphylococcus aureus. Of particular interest are secreted virulence factors. However, due to their potential toxicity and specific post-translational processing, virulence factors are difficult targets for heterologous protein production. Purified proteins with native conformation and adequate purity can therefore often only be achieved by elaborate multi-step purification workflows. While homologous expression in S. aureus theoretically offers a promising alternative in this regard, its application remains limited due to the lack of systems that ensure both tightly controlled expression and subsequent efficient purification.

SUBMITTER: Wolfgramm H 

PROVIDER: S-EPMC12090601 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature