4D quantitative proteomics of ovarian granulosa cells reveals the involvement of oxidative phosphorylation in non-elderly women with diminished ovarian reserve.

ABSTRACT:

Objective

This study compared the proteomic differences between non-elderly diminished ovarian reserve (DOR, < 35 years) and normal ovarian reserve (NOR) to better understand the molecular mechanisms behind ovarian reserve changes in Poseidon Group III.

Methods

Ovarian granulosa cells (GCs) from infertile women with DOR in Poseidon Group III and women with NOR were analyzed using 4D label-free quantitative proteomics. A comprehensive bioinformatics analysis was performed to identify differentially expressed proteins (DEPs) in order to gain a deeper understanding of the mechanisms underlying DOR. The results were subsequently validated by RT-qPCR and Western blot.

Results

In this study, a total of 4,940 proteins were identified. Compared to the NOR group, the non-elder

SUBMITTER: Li HX 

PROVIDER: S-EPMC12090654 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature