KLRG1 identifies regulatory T cells with mitochondrial alterations that accumulate with aging.

ABSTRACT: Recent studies using single-cell RNA sequencing technology have uncovered several subpopulations of CD4⁺ T cells that accumulate with aging. These age-associated T cells are emerging as relevant players in the onset of inflammaging and tissue senescence. Here, based on information provided by single-cell RNA sequencing data, we present a flow cytometry panel that allows the identification of age-associated T cell subsets in systematic larger analysis in mice. We use this panel to evaluate at the single-cell level mitochondrial and senescence marks in the different age-associated CD4⁺ T cell subpopulations. Our analysis identifies a subpopulation of regulatory T (T_reg) cells that is characterized by the extracellular expression of the co-inhibitory molecule

SUBMITTER: Soto-Heredero G 

PROVIDER: S-EPMC12092250 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature