Epidemiology and resistance mechanisms of tigecycline- and carbapenem-resistant <i>Enterobacteriaceae</i> in China: a multicentre genome-based study.

ABSTRACT:

Objectives

To elucidate the molecular epidemiology of tigecycline and carbapenem-resistant Enterobacteriaceae isolates and mechanisms of tigecycline resistance.

Methods

We gathered 31 unduplicated strains of tigecycline-resistant Enterobacteriaceae from six hospitals nationwide. Antimicrobial susceptibility testing, phenotypic detection, and PCR identification were performed first, followed by homology analysis using MLST and PFGE. Conjugation transfer experiments using resistance gene plasmids were carried out, and the conjugates' growth curves were examined. All strains were sequenced using the Illumina HiSeq technology, and we identified a strain KP28 carrying a complete gene cluster tmexCD2-toprJ2. Then, its plasmid was further constructed using the

SUBMITTER: Yan L 

PROVIDER: S-EPMC12092438 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature