Glabridin Hypnosis in Zebrafish Larvae Is Associated With Effects on Multiple Anesthetic Target Receptors.

ABSTRACT:

Background

R -Glabridin is a major flavonoid of licorice ( Glycyrrhiza glabra ) root and known to modulate GABA A receptors, which are targets of many clinical hypnotics. However, R -glabridin hypnotic activity has not been reported in animals.

Methods

Inverted photomotor responses (IPMRs) were used to assess the hypnotic effects of natural R -glabridin and synthetic R/S -glabridin in wild-type zebrafish larvae and transgenic larvae lacking functional GABA A receptor β3 subunits (β3 0/0 ). Two-electrode voltage-clamp electrophysiology in Xenopus oocytes heterologously expressing ion channels quantified the effects of R -glabridin on wild-type and mutated human α1β3γ2L GABA A receptors, NR1B/NR2A N-methyl-D-aspatate (NMDA) receptors, and α4β2 neuronal nicotinic (nnACh) recept

SUBMITTER: Avancha A 

PROVIDER: S-EPMC12092730 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature