Punica granatum leaf extract as a natural antibacterial agent explored by experimental and computational methods.

ABSTRACT: The rise of multidrug-resistant (MDR) microorganisms, particularly Escherichia coli, poses a significant threat to global public health, necessitating the development of alternative treatments. This study investigates the antibacterial properties of methanolic Punica granatum (pomegranate) leaf extract (MPGL) against MDR E. coli and explores its potential as a source of natural inhibitors for the CTX-M-9 beta-lactamase enzyme, a key contributor to antibiotic resistance. The disc diffusion assay revealed that MPGL exhibited significant antibacterial activity, with the highest inhibition zone of 16.67 ± 0.29 mm at 100 µg/disc. Additionally, an in silico approach was employed to identify potential inhibitors from P. granatum phytochemicals. Molecular docking studies demonstrated strong bindin

SUBMITTER: Tasnim F 

PROVIDER: S-EPMC12092750 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature