Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants.

ABSTRACT: The Cucurbitaceae family includes a wide range of economically important fruits and vegetables; however, the laborious and highly inefficient genetic transformation efficacy of cucurbits has hindered the exploration of their gene functions. Virus-induced gene silencing (VIGS) technology, employed from the antiviral RNA silencing defense, has emerged as a viable alternative for high-throughput study of plant gene function. In this study, we successfully established a VIGS system utilizing Trichosanthes mottle mosaic virus (TrMMV), a new member of the genus Tobamovirus. We demonstrated the high efficacy and durability of gene silencing mediated by the TrMMV-VIGS vector in Nicotiana benthamiana, as well as in several cucurbit species, including Cucurbita pepo, Cucumis sativus, C. lanatus, and

SUBMITTER: Chen C 

PROVIDER: S-EPMC12092892 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature