Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer.

ABSTRACT:

Purpose

Radiation therapy has revolutionized the treatment of primary or liver metastases in colorectal cancer (CRC). In colorectal cancer, conventional fractionation (1.8 ~ 2.0 Gy daily) is typically used for treatment. Nevertheless, there is a paucity of research investigating the potential implications of radiation therapy-induced alterations in the expression levels of regulatory genes on resistance to chemotherapy agents. Herein, we explored the mechanism by which conventional fractionation drives 5-fluorouracil (5-FU) resistance and metformin (Met) rescued 5-FU resistance in CRC.

Methods and materials

RNA sequencing, differential genes expression analysis was performed to identify the 5-FU resistance genes after irradiation (according to the convention of cell irradiat

SUBMITTER: Wang S 

PROVIDER: S-EPMC12093704 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature