Rapid visual detection of Monkeypox virus by one-step LAMP-CRISPR/Cas12b assay.

ABSTRACT:

Background

Monkeypox virus (MPXV) infection has garnered significant global attention due to its rising incidence and substantial public health implications. A rapid, sensitive, and accurate diagnostic method is urgently required to enable early intervention and effective management of MPXV outbreaks.

Results

In this study, we developed a novel one-step assay that integrates loop-mediated isothermal amplification (LAMP) with CRISPR/Cas12b in one-pot for the detection of MPXV. The entire detection process did not require opening the lid of the reaction tube and could be completed within 40 min using extracted viral nucleic acids, which is faster than real-time quantitative PCR (qPCR). And the results could be interpreted through either real-time fluorescence or naked-eye visu

SUBMITTER: Guo J 

PROVIDER: S-EPMC12093717 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature