Identification of a critical gene involved in the biosynthesis of the polyene macrolide lavencidin in <i>Streptomyces lavendulae</i> FRI-5 using the Target-AID (activation-induced cytidine deaminase) base editing technology.

ABSTRACT: Polyene macrolide antibiotics, produced mainly as secondary metabolites of streptomycetes, have distinct chemical structures and include clinically important antifungal drugs. We recently isolated the 28-membered polyene macrolide lavencidin from Streptomyces lavendulae FRI-5. Here, we identify and characterize the lavencidin biosynthetic (lad) gene cluster by combining a gene disruption system based on a base editing technology and in silico analysis. Sequence analysis of the draft genome of S. lavendulae FRI-5 revealed plausible lavencidin biosynthetic genes, which could be assigned roles in the biosynthesis of the polyketide backbone and the peripheral moiety, as well as in the regulation of lavencidin production. The introduction of a stop codon into the

SUBMITTER: Otsuka R 

PROVIDER: S-EPMC12093942 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature