Construction of an efficient electroporation transformation system promotes the application of Targetron in wild-type <i>Paenibacillus elgii</i> 219.

ABSTRACT: Paenibacillus species have attracted much attention as a promising cell factory that can yield a variety of bioactive peptides and functional exopolysaccharides. Improving the production capacity of Paenibacillus through genetic engineering is crucial for the development of bioactive natural products. This study systematically evaluated the effects of electroporation transformation parameters on the introduction of DNA into Paenibacillus elgii 219, which was isolated from soil and preserved in our lab. The transformation efficiency was improved to 1.25 × 10⁶ transformants/μg DNA by optimizing factors including the growth and recovery media content, the growth stages of P. elgii 219, electric field strength, electroporation buffer composition, cell wal

SUBMITTER: Yang G 

PROVIDER: S-EPMC12093971 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature