Engineering the hyperthermophilic archaeon <i>Pyrococcus furiosus</i> for 1-propanol production.

ABSTRACT: Society relies heavily on chemicals traditionally produced through the refinement of fossil fuels. The conversion of renewable biomass to value-added chemicals by microbes, particularly hyperthermophiles (T_opt ≥80°C), offers a renewable alternative to this traditional approach. Herein, we describe the engineering of the hyperthermophilic archaeon Pyrococcus furiosus, which grows optimally (T_opt) at 100°C, for the conversion of sugar to 1-propanol. This was accomplished by constructing a hybrid metabolic pathway consisting of two native and seven heterologously produced enzymes to convert acetyl-CoA from carbohydrate metabolism to 1-propanol. A total of eleven foreign genes from two other organisms were utilized, one from the thermophilic bacterium Thermoanae

SUBMITTER: O'Quinn HC 

PROVIDER: S-EPMC12094016 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature